bim antibody Search Results


bim antibody  (Novus Biologicals)
90
Novus Biologicals bim antibody
Bim Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pm17659279-55-53-55?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
bim antibody - by Bioz Stars, 2026-07
90/100 stars
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bim  (Novus Biologicals)
93
Novus Biologicals bim
Bim, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pmc02819752-121-43-44?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
bim - by Bioz Stars, 2026-07
93/100 stars
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bim  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology bim
Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated <t>BIM</t> and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression <t>of</t> <t>Bcl-2</t> family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.
Bim, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pm32133047-186-13-31?v=Santa+Cruz+Biotechnology
Average 95 stars, based on 1 article reviews
bim - by Bioz Stars, 2026-07
95/100 stars
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bim  (ProSci Incorporated)
90
ProSci Incorporated bim
Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated <t>BIM</t> and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression <t>of</t> <t>Bcl-2</t> family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.
Bim, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pmc05852046-230-22-25?v=ProSci+Incorporated
Average 90 stars, based on 1 article reviews
bim - by Bioz Stars, 2026-07
90/100 stars
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bim  (Proteintech)
93
Proteintech bim
Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated <t>BIM</t> and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression <t>of</t> <t>Bcl-2</t> family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.
Bim, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pmc12911246-93-66-68?v=Proteintech
Average 93 stars, based on 1 article reviews
bim - by Bioz Stars, 2026-07
93/100 stars
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anti bim  (Rockland Immunochemicals)
85
Rockland Immunochemicals anti bim
Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated <t>BIM</t> and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression <t>of</t> <t>Bcl-2</t> family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.
Anti Bim, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pmc04160434-30-16-13?v=Rockland+Immunochemicals
Average 85 stars, based on 1 article reviews
anti bim - by Bioz Stars, 2026-07
85/100 stars
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rabbit polyclonal antibodies against bim  (Novus Biologicals)
90
Novus Biologicals rabbit polyclonal antibodies against bim
Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated <t>BIM</t> and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression <t>of</t> <t>Bcl-2</t> family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.
Rabbit Polyclonal Antibodies Against Bim, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/pm23087082-70-0-7?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies against bim - by Bioz Stars, 2026-07
90/100 stars
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rabbit polyclonal anti bim  (Novus Biologicals)
91
Novus Biologicals rabbit polyclonal anti bim
Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated <t>BIM</t> and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression <t>of</t> <t>Bcl-2</t> family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.
Rabbit Polyclonal Anti Bim, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bim+antibody/ppr0621315-86-116-122?v=Novus+Biologicals
Average 91 stars, based on 1 article reviews
rabbit polyclonal anti bim - by Bioz Stars, 2026-07
91/100 stars
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Image Search Results


Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated BIM and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression of Bcl-2 family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.

Journal: Oncotarget

Article Title: MAGE-A inhibit apoptosis and promote proliferation in multiple myeloma through regulation of BIM and p21 Cip1 .

doi: 10.18632/oncotarget.27488

Figure Lengend Snippet: Figure 2: Silencing of MAGE-A in HMCL results in stabilization of phosphorylated BIM and p21Cip1. MM.1r and H929 were transduced with MAGE-A3-specific lentiviral shRNA constructs or controls as described, and heavy membrane preps (A, C) were probed for expression of Bcl-2 family proteins. (A) Western blot of heavy membrane preps for Bcl-2 family proteins. MAGE-A3 knockdown and increased p53 protein were confirmed by western blot of whole cell lysate. COXIV, load control, representative of all blots. (B) Optical densitometry of western blots for BIM el as in (A) demonstrates significantly higher levels of this protein after MAGE-A silencing compared to controls. Data was pooled from five replicates, representing 13–15 data points each. Error bars, standard error of the mean. (C) Western blot of heavy membrane preps for p-ser69 and p-ser77 Bim el demonstrate stabilization of the phosphorylated protein after MAGE-A knockdown. (D) Whole cell lysates were probed by western blot for p21Cip1. (E) Optical densitometry of western blots for p21Cip1 as in (D) demonstrates significantly higher levels after MAGE-A silencing. Data pooled from five replicates, representing 15 data points each. Con, Untreated control cells. shNT, non-target shRNA lentiviral construct. shMA, MAGE-A3 targeted lentiviral shRNA construct TRCN0000128375.

Article Snippet: The antibodies used were: Bcl-2 (Cell Signaling Technologies, 2872), Bcl-xL (2762), BID (2002), BIM (2819), CoxIV (4850), Mcl-1 (4572), pSer69-BIM (4585), pSer77-BIM (12433), p21 (2947), BMF (Abcam, ab181148), GAPDH (ab8245), Bcl-G (Santa Cruz, sc-393715), Caspase 3 Oncotarget737www.oncotarget.com (sc-7272), MAGE6C1 (sc-20034), PUMA (sc-374223), p53 (sc-126), goat anti-mouse IgG HRP conjugate (Thermo Scientific, 32430) and goat anti-rabbit IgG HRP conjugate (32460).

Techniques: Transduction, shRNA, Construct, Membrane, Expressing, Western Blot, Knockdown, Control

Figure 5: Model of MAGE-A3 activity in MM. (A) DNA damage response pathways promote BIM expression and p53 transcriptional activity, resulting in increased expression of BAX and p21Cip1. BIM displaces BAX from anti-apoptotic Bcl-2 family proteins, allowing it to translocate to mitochondrial membranes and initiate apoptosis. P21Cip1 inhibits cyclin D1/CDK4/6 activity, blocking progression through the early G1 checkpoint. (B) MAGE-A3/RING complex activity, possibly activated through interactions with DNA repair pathways, down- regulates BIM and p53 through transcriptional and post-translational mechanisms, resulting in survival and proliferation.

Journal: Oncotarget

Article Title: MAGE-A inhibit apoptosis and promote proliferation in multiple myeloma through regulation of BIM and p21 Cip1 .

doi: 10.18632/oncotarget.27488

Figure Lengend Snippet: Figure 5: Model of MAGE-A3 activity in MM. (A) DNA damage response pathways promote BIM expression and p53 transcriptional activity, resulting in increased expression of BAX and p21Cip1. BIM displaces BAX from anti-apoptotic Bcl-2 family proteins, allowing it to translocate to mitochondrial membranes and initiate apoptosis. P21Cip1 inhibits cyclin D1/CDK4/6 activity, blocking progression through the early G1 checkpoint. (B) MAGE-A3/RING complex activity, possibly activated through interactions with DNA repair pathways, down- regulates BIM and p53 through transcriptional and post-translational mechanisms, resulting in survival and proliferation.

Article Snippet: The antibodies used were: Bcl-2 (Cell Signaling Technologies, 2872), Bcl-xL (2762), BID (2002), BIM (2819), CoxIV (4850), Mcl-1 (4572), pSer69-BIM (4585), pSer77-BIM (12433), p21 (2947), BMF (Abcam, ab181148), GAPDH (ab8245), Bcl-G (Santa Cruz, sc-393715), Caspase 3 Oncotarget737www.oncotarget.com (sc-7272), MAGE6C1 (sc-20034), PUMA (sc-374223), p53 (sc-126), goat anti-mouse IgG HRP conjugate (Thermo Scientific, 32430) and goat anti-rabbit IgG HRP conjugate (32460).

Techniques: Activity Assay, Expressing, Blocking Assay